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Proc Natl Acad Sci U S A. 2011 Apr 19;10816:6626 31. Epub 2011 Apr 4. Hyperactivation of anandamide synthesis and regulation of cell cycle progression via cannabinoid type 1 CB1 receptors in the regenerating liver. The mammalian liver regenerates upon tissue loss, which induces quiescent hepatocytes case study enter the cell cycle and undergo restricted replication under the manage of assorted hormones, increase elements, and cytokines. Endocannabinoids acting via cannabinoid type 1 receptors CB1R advertise neural progenitor cell proliferation, and in the liver they advertise lipogenesis. These findings suggest the involvement of CB1R in the handle of liver regeneration. In the latest study, researchers record that mice missing CB1R globally or in hepatocytes only and wild type mice dealt with with a CB1R antagonist have a delayed proliferative response case study two thirds partial hepatectomy PHX. In wild type mice, PHX leads case study higher hepatic expression of CB1R and hyperactivation of the biosynthesis of the endocannabinoid anandamide in the liver via an in vivo pathway regarding conjugation of arachidonic acid and ethanolamine by fatty acid amide hydrolase. In wild type but not CB1R / mice, PHX induces robust up regulation of key cell cycle proteins involved in mitotic development, including cyclin dependent kinase 1 Cdk1, cyclin B2, and their transcriptional regulator forkhead box protein M1 FoxM1, as discovered by ultrahigh throughput RNA sequencing and pathway evaluation and confirmed by real time PCR and Western blot analyses. Treatment of untamed type mice with anandamide induces similar adjustments mediated via activation of the PI3K/Akt pathway.
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